Downstream Bench Scientist
MilliporeSigma

Burlington, Massachusetts

Posted in Science and Research


This job has expired.

Job Info


A career with MilliporeSigma is an ongoing journey of discovery: our 57,000 people are shaping how the world lives, works and plays through next generation advancements in healthcare, life science and electronics. For more than 350 years and across the world we have passionately pursued our curiosity to find novel and vibrant ways of enhancing the lives of others. MilliporeSigma is a business of Merck KGaA, Darmstadt, Germany.

Your Role:

MilliporeSigma in Burlington, MA is seeking a Downstream Bench Scientist to assist with design fit-for-purpose purification experiments according to the stage of process development. In this role, the Scientist will be working with client products in a CDMO environment.

Responsibilities:

  • Ensure scientific evaluation of impact of deviations to Downstream validation studies
  • Compile and analyze experimental data to generate process knowledge and decide on next steps
  • Write development and validation reports and ensure their timely delivery
  • Prepare and present project updates to global project team and to upper management
  • Responsible for activities ranging from protein purification, filter sizing studies, biochemical and biophysical characterization of proteins (mAb, Fc-fusion, bispecifics and enzymes)
  • Execute routine protein purification with affinity, size exclusion, ion exchange, and hydrophobic interaction chromatography using AKTA systems (Unicorn software)
  • Perform analyses including but not limited to SDS-PAGE, SE-HPLC, LAL, host cell-DNA, host cell protein, as necessary
  • Provide expertise and insight to guide development of appropriate purification methods
  • Develop novel purification processes as well as using standard methods to purify proteins from 1 mg to 200-gram scale. Processes will be multiple steps and must provide necessary clearance of viruses, endotoxin, host cell proteins and DNA
  • Perform, develop, and optimize robust, efficient, scalable strategies for improving process performance. Activities will include small-scale studies and pilot scale purification studies.

Who You Are:

Minimum Qualifications:
  • Bachelor's degree in Biology, Biochemistry, or other Scientific discipline with 5+ years' experience in protein purification and/or formulation study or Master's degree in Biology, Biochemistry, or other Scientific discipline with 2+ years' experience in protein purification and/or formulation study

Preferred Qualifications:
  • PhD in Biotechnology with experience in protein purification. Master's Degree in biotechnology or Life Science with 5+ years in the biopharmaceutical downstream process development experience
  • Solid and proven theoretical basis and hands-on experience in protein purification in process development and analytical characterization
  • Hands-on experience with purification unit operations including filtration, chromatography, TFF, UF/DF, AKTA, UNICORN and HPLC systems
  • Hands-on experience in troubleshooting and routine instrument maintenance
  • Experienced in protein purification via affinity, hydrophobic interaction (HIC), ion exchange (IEX), size exclusion (SEC) and mixed-mode chromatography
  • Knowledge and skills in biochemical characterization of proteins including analytical and biophysical
  • Proven track record in Purification Process Development with emphasis on chromatographic separations and excellent understanding of and experience with chromatographic resin screening and process optimization
  • Solid and proven working knowledge of DOE. Ability to critically analyze data using statistical tools and to compile technical reports.
  • Experience in formulation studies and DOE analysis
  • Experience with scale-up and GMP manufacturing is desirable
  • Excellent presentation and communication skills, both written and oral
  • Results-oriented with ability to work and deliver under pressure

What we offer: With us, there are always opportunities to break new ground. We empower you to fulfil your ambitions, and our diverse businesses offer various career moves to seek new horizons. We trust you with responsibility early on and support you to draw your own career map that is responsive to your aspirations and priorities in life. Join us and bring your curiosity to life!

Curious? Apply and find more information at https://jobs.vibrantm.com

The Company is an Equal Employment Opportunity employer. No employee or applicant for employment will be discriminated against on the basis of race, color, religion, age, sex, sexual orientation, national origin, ancestry, disability, military or veteran status, genetic information, gender identity, transgender status, marital status, or any other classification protected by applicable federal, state, or local law. This policy of Equal Employment Opportunity applies to all policies and programs relating to recruitment and hiring, promotion, compensation, benefits, discipline, termination, and all other terms and conditions of employment. Any applicant or employee who believes they have been discriminated against by the Company or anyone acting on behalf of the Company must report any concerns to their Human Resources Business Partner, Legal, or Compliance immediately. The Company will not retaliate against any individual because they made a good faith report of discrimination.

Job Requisition ID: 218704

Location: Burlington

Career Level: C - Professional (1-3 years)

Working time model: full-time


This job has expired.

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