GENERAL SUMMARY/OVERVIEW STATEMENT:
The self-motivated applicant will work in the Dettmer Lab within the Department of Neurology, Ann Romney Center for Transformative Research, Brigham and Women's Hospital. This group studies the basis of neurological disorders such Parkinson's disease, Lewy Body Dementia and Multiple System Atrophy using techniques in cell biology, biochemistry, high-content imaging, microscopy, human genetics and lipid biology. The position will focus on understanding the molecular basis of neurological disorders. The ideal candidate will at first work under close supervision and then more and more independently. She/he will execute protocols involving a variety of routine and non-routine experiments at a professional level. The qualified candidate will develop modification of existing techniques, as appropriate. In collaboration with other members of the research team, he/she will give more and more input to research design and will calculate, transcribe and graph data. The position offers a unique opportunity to expand research methodologies within the rich scientific community of Brigham and Women's Hospital and Harvard Medical School.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
- Under general supervision, perform basic cell culture, primary neuron culture, iPSC culture and in vitro assays and examination of protein expression level by ELISA.
- Cell maintenance (human and rodent).
- Knockdown and over-expression of target genes in mammalian models.
- Drug treatments of multiple cell types.
- End point assays (LDH, Adenylate kinase etc), analysis using commercial kits.
- Virus preparation.
- Protein isolation, quantification (BCA) and western blots.
- General molecular biology e.g. cloning, plasmid preps, genomic DNA and RNA isolation, PCR.
- Should be able to work with animals (rodents).
- Coordinates daily schedule of experiments.
- Responsible for data management: collects, records, tabulates and presents data. Creates graphs of experimental, performs statistical tests, and discusses progress of work regularly with supervisor.
- All other duties as assigned.
- Must have a Bachelor's degree.
- Ideally has relevant experience in wet labs and familiarity with cell line/iPSC work (preferred in sections mentioned in "principal duties and responsibilities").
- High degree of computer literacy.
- Sound analytical and organizational skills.
- Good oral and written communication skills.
- Structures logically and effectively tasks and set priorities.
- Is able to identify potential problems and trouble-shoot solutions.
- Demonstrates ability to analyze data statistically and to logically present it.
- Shows strong interpersonal and communication skills.
- Normal laboratory environment.
- Hazardous materials are used in some of the experiments conducted.
- Responsible for training and orienting new staff. May serve as a team leader or supervisor in a smaller research setting.
Brigham and Women's Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, creed, sex, sexual orientation, gender identity, national origin, ancestry, age, veteran status, disability unrelated to job requirements, genetic information, military service, or other protected status.